NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 3D TROSY HNCA | 0.8 mM [U-13C; U-15N; 85%-2H] HIV-1 gp41 Transmembrane Domain, 60 mM regular 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM regular 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |
2 | 3D TROSY HNCOCA | 0.8 mM [U-13C; U-15N; 85%-2H] HIV-1 gp41 Transmembrane Domain, 60 mM regular 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM regular 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |
3 | 3D TROSY HNCACO | 0.8 mM [U-13C; U-15N; 85%-2H] HIV-1 gp41 Transmembrane Domain, 60 mM regular 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM regular 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |
4 | 3D TROSY HNCO | 0.8 mM [U-13C; U-15N; 85%-2H] HIV-1 gp41 Transmembrane Domain, 60 mM regular 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM regular 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |
5 | 3D 15N NOESY-TROSY-HSQC | 0.8 mM [U-13C; U-15N] HIV-1 gp41 Transmembrane Domain, 60 mM acyl chains 2H labeled 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM acyl chains 2H labeled 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE III 900 |
6 | 3D 13C NOESY-HSQC (Methyls) | 0.8 mM [U-13C; U-15N] HIV-1 gp41 Transmembrane Domain, 60 mM acyl chains 2H labeled 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM acyl chains 2H labeled 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE III 900 |
7 | 3D 15N NOESY-TROSY-HSQC (long mixing time) | 0.4 mM [U-15N; U-2H] HIV-1 gp41 Transmembrane Domain, 0.4 mM [15% 13C] HIV-1 gp41 Transmembrane Domain, 60 mM acyl chains 2H labeled 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM acyl chains 2H labeled 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 750 |
8 | 2D 15N TROSY-HSQC for GdDOTA titration | 0.8 mM [U-13C; U-15N; 85%-2H] HIV-1 gp41 Transmembrane Domain, 60 mM regular 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM regular 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |
9 | 3D 15N NOESY-TROSY-HSQC (short mixing time) | 0.8 mM [U-15N] HIV-1 gp41 Transmembrane Domain, 60 mM acyl chains 2H labeled 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC), 120 mM acyl chains 2H labeled 1,2-Dihexanoyl-sn-Glycero-3-Phosphocholine (DHPC), 25 mM regular MES, 0.1 % regular NaN3 | 95% H2O/5% D2O | 50 mM | 6.7 | 1 atm | 303 | Bruker AVANCE II 600 |