5ION
Solution Structure of Miz-1 Zinc Finger 13
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H NOESY | 0.75 mM 1H ZF 13 | 90% H2O/10% D2O | 0.05 M | 6.5 | 1 atm | 298 | Varian Unity Inova 600 |
| 2 | 2D 1H-1H TOCSY | 0.75 mM 1H ZF 13 | 90% H2O/10% D2O | 0.05 M | 6.5 | 1 atm | 298 | Varian Unity Inova 600 |
| 3 | 2D 1H-13C HSQC aliphatic | 0.75 mM 1H ZF 13 | 90% H2O/10% D2O | 0.05 M | 6.5 | 1 atm | 298 | Varian Unity Inova 600 |
| 4 | 2D 1H-13C HSQC aromatic | 0.75 mM 1H ZF 13 | 90% H2O/10% D2O | 0.05 M | 6.5 | 1 atm | 298 | Varian Unity Inova 600 |
| 5 | 2D 1H-15N HSQC | 0.75 mM 1H ZF 13 | 90% H2O/10% D2O | 0.05 M | 6.5 | 1 atm | 298 | Varian Unity Inova 600 |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | Unity Inova | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 300 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | NMRPipe | 7.4 | Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax |
| 2 | chemical shift assignment | CcpNmr Analysis | CCPN | |
| 3 | data analysis | DANGLE | 1.1 | CCPN |
| 4 | refinement | CNS | 1.21 | Brunger, Adams, Clore, Gros, Nilges and Read |
| 5 | structure calculation | ARIA | 2.2 | Linge, O'Donoghue and Nilges |
