Experiment: 5H7U

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-15N HSQC	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 800
2	3D HNCO	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
3	3D HN(CA)CO	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
4	3D CBCA(CO)NH	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
5	3D HNCACB	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
6	3D C(CO)NH	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
7	3D HBHA(CO)NH	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
8	2D 1H-13C HSQC	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 800
9	3D HCCH-TOCSY	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
10	3D HCCH-COSY	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 600
11	3D 1H-15N NOESY	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 800
12	3D 1H-13C NOESY	0.4 mM [U-100% 13C; U-100% 15N] eIF3c 36-163, 20 mM sodium phosphate, 150 mM sodium chloride, 1 mM DTT	95% H2O/5% D2O	170 mM	7	1 atm	298	Bruker AVANCE 800

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	800
2	Bruker	AVANCE	600

NMR Refinement
Method	Details	Software
DGSA-distance geometry simulated annealing		Amber

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	200
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	Amber	9	Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman
2	structure calculation	CYANA	2.1	Guntert, Mumenthaler and Wuthrich
3	chemical shift assignment	KUJIRA	0.98	Naohiro Kobayashi
4	peak picking	NMRView		Johnson, One Moon Scientific
5	processing	NMRPipe		Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.