5G04
Structure of the human APC-Cdc20-Hsl1 complex
ELECTRON MICROSCOPY
| Sample |
|---|
| RECOMBINANT APC-CDC20- HSL1 COMPLEX |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK III |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | LIQUID ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 179660 |
| Reported Resolution (Å) | 3.9 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | HALF-MAPS REFINED INDEPENDENTLY |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI FALCON II (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 27 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2015-10-30 |
| Temperature (Kelvin) | 100 |
| Microscope Model | FEI POLARA 300 |
| Minimum Defocus (nm) | 2000 |
| Maximum Defocus (nm) | 4000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 78000 |
| Calibrated Magnification | 78000 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
