5APN

Structure of the yeast 60S ribosomal subunit in complex with Arx1, Alb1 and N-terminally tagged Rei1


ELECTRON MICROSCOPY
Sample
YEAST 60S ARX1 ALB1 6XHIS-REI1
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE-PROPANE
Sample Vitrification DetailsPLUNGE-FROZEN
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles22040
Reported Resolution (Å)3.91
Resolution Method
Other DetailsFOR VISUALIZATION PURPOSES THE FINAL MAP WAS FILTERED AND AMPLITUDE CORRECTED IN RELION. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-315 ...FOR VISUALIZATION PURPOSES THE FINAL MAP WAS FILTERED AND AMPLITUDE CORRECTED IN RELION. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3152 (DEPOSITION ID 13746). THE COORDINATES WERE REFINED IN RECIPROCAL SPACE USING PHENIX.REFINE AGAINST THE MLHL TARGET. FOR THIS, THE CRYO-EM MAP WAS CONVERTED TO RECIPROCAL SPACE STRUCTURE FACTORS.
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2015-04-17
Temperature (Kelvin)80
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1000
Maximum Defocus (nm)3000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification59000
Calibrated Magnification100720
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging DetailsCOLLECTED IN MOVIE MODE
EM Software
TaskSoftware PackageVersion
CTF CORRECTIONCTFFIND3
IMAGE ACQUISITIONEPU
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONRELION1.3
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PER DETECTOR FRAME