5AHV

Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes


ELECTRON MICROSCOPY
Sample
ANTH AND ENTH DOMAINS OF THE CLATHRIN ADAPTORS SLA2 AND ENT1, RESPECTIVELY
Specimen Preparation
Sample Aggregation StateFILAMENT
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification DetailsVITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 80, INSTRUMENT- HOMEMADE PLUNGER, METHOD- , APPLIED ON C-FLAT HOLEY CARBON COATED GRIDS (PROTOCHIPS) AND ...VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 80, INSTRUMENT- HOMEMADE PLUNGER, METHOD- , APPLIED ON C-FLAT HOLEY CARBON COATED GRIDS (PROTOCHIPS) AND VITRIFIED,
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles457344
Reported Resolution (Å)13.6
Resolution Method
Other DetailsSUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2896. (DEPOSITION ID: 13053).
Refinement Type
Symmetry TypeHELICAL
Map-Model Fitting and Refinement
Id1 (1H0A)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--FLEXIBLE REFINEMENT PROTOCOL--HOMOLOGY MODEL
Data Acquisition
Detector TypeGATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å**2)10
Imaging Experiment1
Date of Experiment2013-03-01
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)500
Maximum Defocus (nm)5000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification59000
Calibrated Magnification59000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)120
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGDireX
MODEL FITTINGI-TASSER
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONSPRING
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
EACH PARTICLE