5AFU
Cryo-EM structure of dynein tail-dynactin-BICD2N complex
ELECTRON MICROSCOPY
| Sample |
|---|
| CRYO-EM STRUCTURE OF DYNEIN TAIL-DYNACTIN- BICD2N COMPLEX |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK III |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | LIQUID ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 85744 |
| Reported Resolution (Å) | 8.2 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | HALF-MAPS REFINED INDEPENDENTLY |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI FALCON II (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 2 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2014-09-12 |
| Temperature (Kelvin) | 100 |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 3000 |
| Maximum Defocus (nm) | 8000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 47000 |
| Calibrated Magnification | 82353 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
