4AN5

Capsid structure and its Stability at the Late Stages of Bacteriophage SPP1 Assembly

ELECTRON MICROSCOPY

Sample
CAPSID OF SPP1

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK I
Cryogen Name	ETHANE
Sample Vitrification Details	VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- FEI VITROBOT MARK I,

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	5000
Reported Resolution (Å)	8.8
Resolution Method
Other Details	ANGULAR RECONSTITUTION. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2049. (DEPOSITION ID: 10649).
Refinement Type
Symmetry Type	POINT
Point Symmetry	I

Map-Model Fitting and Refinement
Id	1 (1OHG, 1YUE)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
Details	METHOD--EACH HELIX AND EVERY BETA SHEET WERE TREATED AS RIGID BODIES.

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment	2005-04-06
Temperature (Kelvin)
Microscope Model	JEOL 2200FS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	50000
Calibrated Magnification
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details	LOW-DOSE IMAGING PROCEDURE

EM Software
Task	Software Package	Version
MODEL FITTING	Flex-EM
RECONSTRUCTION	IMAGIC

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	PHASE FLIPPING

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.