4AN5
Capsid structure and its Stability at the Late Stages of Bacteriophage SPP1 Assembly
ELECTRON MICROSCOPY
Sample |
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CAPSID OF SPP1 |
Specimen Preparation | |
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Sample Aggregation State | PARTICLE |
Vitrification Instrument | FEI VITROBOT MARK I |
Cryogen Name | ETHANE |
Sample Vitrification Details | VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- FEI VITROBOT MARK I, |
3D Reconstruction | |
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Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 5000 |
Reported Resolution (Å) | 8.8 |
Resolution Method | |
Other Details | ANGULAR RECONSTITUTION. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2049. (DEPOSITION ID: 10649). |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | I |
Map-Model Fitting and Refinement | |||||
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Id | 1 (1OHG, 1YUE) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | RIGID BODY FIT | ||||
Refinement Target | |||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | METHOD--EACH HELIX AND EVERY BETA SHEET WERE TREATED AS RIGID BODIES. |
Data Acquisition | |||||||||
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Detector Type | KODAK SO-163 FILM | ||||||||
Electron Dose (electrons/Å**2) | 20 |
Imaging Experiment | 1 |
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Date of Experiment | 2005-04-06 |
Temperature (Kelvin) | |
Microscope Model | JEOL 2200FS |
Minimum Defocus (nm) | |
Maximum Defocus (nm) | |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | 50000 |
Calibrated Magnification | |
Source | FIELD EMISSION GUN |
Acceleration Voltage (kV) | 200 |
Imaging Details | LOW-DOSE IMAGING PROCEDURE |
EM Software | ||
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Task | Software Package | Version |
MODEL FITTING | Flex-EM | |
RECONSTRUCTION | IMAGIC |
Image Processing | ||||
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CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
PHASE FLIPPING |