4V69
Ternary complex-bound E.coli 70S ribosome.
ELECTRON MICROSCOPY
| Sample |
|---|
| E. coli ribosome in complex with the ternary complex EF-Tu aminoacyl-tRNA GDP |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK I |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Vitrification using FEI Vitrobot; blot 3 seconds before plunging with an offset of -1mm |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 131599 |
| Reported Resolution (Å) | 6.7 |
| Resolution Method | |
| Other Details | see LeBarron et al., JSB 164 (2008) 24-32. |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2I2U, 2I2V, 2J00, 1OB2) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | Cross correlation, root-mean square deviation | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--See Trabuco et al., Structure 16 (2008) 673-683. REFINEMENT PROTOCOL--Flexible fitting | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 20 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2006-08-31 |
| Temperature (Kelvin) | 84 |
| Microscope Model | FEI POLARA 300 |
| Minimum Defocus (nm) | 4520 |
| Maximum Defocus (nm) | 1200 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 59000 |
| Calibrated Magnification | 58279 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | MDFF | |
| RECONSTRUCTION | SPIDER | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| Correction of reconstruction of each defocus group | ||||
