4UQQ

Electron density map of GluK2 desensitized state in complex with 2S,4R-4-methylglutamate


ELECTRON MICROSCOPY
Sample
GLUK2
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsETHANE
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles21360
Reported Resolution (Å)7.6
Resolution Method
Other DetailsCOORDINATES FROM 3KG2 WERE FIT AS SEVEN INDEPENDENT RIGID BODIES CONSISTING OF TWO ATD DIMERS FROM PDB ID 3H6G FOUR LBD MONOMERS FROM PDB 3G3F AND A T ...COORDINATES FROM 3KG2 WERE FIT AS SEVEN INDEPENDENT RIGID BODIES CONSISTING OF TWO ATD DIMERS FROM PDB ID 3H6G FOUR LBD MONOMERS FROM PDB 3G3F AND A TMD TETRAMER FROM PDB 3KG2 GEOMETRY AND STEREOCHEMISTRY OUTLIERS ARE THOSE PRESENT IN PDB 3H6G 3G3F AND 3KG2 USED FOR RIGID BODY FITS SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2685. (DEPOSITION ID: 12610).
Refinement Type
Symmetry TypePOINT
Point SymmetryC2
Map-Model Fitting and Refinement
Id1 (3H6G, 3G3F, 3KG2)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)25
Imaging Experiment1
Date of Experiment2013-08-01
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)2000
Maximum Defocus (nm)3500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification47000
Calibrated Magnification47000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONRELION
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
EACH PARTICLE