4UJE

Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement

ELECTRON MICROSCOPY

Sample
POST-TRANSLOCATIONAL RABBIT 80S RIBOSOME WITH TWO TRNAS AND MRNA BOUND

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK II
Cryogen Name	ETHANE
Sample Vitrification Details	VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 80, INSTRUMENT- FEI VITROBOT MARK II, METHOD- BLOT FOR 2 SECONDS BEFORE PLUNGING

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	236113
Reported Resolution (Å)	6.9
Resolution Method
Other Details	SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2620. (DEPOSITION ID: 12402).
Refinement Type
Symmetry Type	POINT
Point Symmetry	C1

Map-Model Fitting and Refinement
Id	1
Refinement Space	REAL
Refinement Protocol	FLEXIBLE FIT
Refinement Target	Cross-correlation coefficient
Overall B Value
Fitting Procedure
Details	METHOD--MDFIT

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment	2008-06-02
Temperature (Kelvin)
Microscope Model	FEI TECNAI F30
Minimum Defocus (nm)	2000
Maximum Defocus (nm)	4000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	39000
Calibrated Magnification	65520
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details	MINIMAL DOSE SYSTEM

EM Software
Task	Software Package	Version
RECONSTRUCTION	SPARX
RECONSTRUCTION	SPIDER

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	DEFOCUS GROUP

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.