4CSU
Cryo-EM structures of the 50S ribosome subunit bound with ObgE
ELECTRON MICROSCOPY
Sample |
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50S RIBOSOME BOUND WITH OBGE |
Specimen Preparation | |
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Sample Aggregation State | PARTICLE |
Vitrification Instrument | FEI VITROBOT MARK IV |
Cryogen Name | ETHANE |
Sample Vitrification Details | LIQUID ETHANE |
3D Reconstruction | |
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Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 102814 |
Reported Resolution (Å) | 5.5 |
Resolution Method | |
Other Details | SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2605. (DEPOSITION ID: 12315) |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | C1 |
Map-Model Fitting and Refinement | |||||
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Id | 1 (3OFC) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | OTHER | ||||
Refinement Target | |||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | REFINEMENT PROTOCOL--X-RAY |
Data Acquisition | |||||||||
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Detector Type | FEI EAGLE (4k x 4k) | ||||||||
Electron Dose (electrons/Å**2) | 20 |
Imaging Experiment | 1 |
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Date of Experiment | 2011-07-27 |
Temperature (Kelvin) | |
Microscope Model | FEI TITAN KRIOS |
Minimum Defocus (nm) | 1000 |
Maximum Defocus (nm) | 4000 |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | 2.7 |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | 59000 |
Calibrated Magnification | |
Source | FIELD EMISSION GUN |
Acceleration Voltage (kV) | 300 |
Imaging Details |
EM Software | ||
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Task | Software Package | Version |
MODEL FITTING | MDFF | |
RECONSTRUCTION | RELION |
Image Processing | ||||
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CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
INDIVIDUAL PARTICLES |