4CBF
Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus
ELECTRON MICROSCOPY
| Sample |
|---|
| MATURE DENGUE VIRUS SEROTYPE 4 |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | LIQUID ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 16602 |
| Reported Resolution (Å) | 4.1 |
| Resolution Method | |
| Other Details | MPSA SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2485. (DEPOSITION ID: 12027). |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (3J27) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | REAL SPACE CORRELATION | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--FLEXIBLE REFINEMENT PROTOCOL--CRYO-EM | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI FALCON I (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 18 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2012-04-14 |
| Temperature (Kelvin) | 100 |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 500 |
| Maximum Defocus (nm) | 3500 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 125000 |
| Calibrated Magnification | 129629 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | CNS | |
| MODEL FITTING | Coot | |
| MODEL FITTING | UCSF Chimera | |
| RECONSTRUCTION | EMAN | 1 |
| RECONSTRUCTION | EMAN | 2 |
| RECONSTRUCTION | MPSA | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| EACH PARTICLE | ||||
