4BOO

The structure and super-organization of acetylcholine receptor-rapsyn complexes class C

ELECTRON MICROSCOPY

Sample
ACETYLCHOLINE RECEPTOR AND RAPSYN

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	HOMEMADE PLUNGER
Cryogen Name	ETHANE
Sample Vitrification Details	CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 78, INSTRUMENT- HOMEMADE PLUNGER, METHOD- BLOT FROM THE CARBON SIDE

3D Reconstruction
Reconstruction Method	TOMOGRAPHY
Number of Particles	3564
Reported Resolution (Å)	42
Resolution Method
Other Details	SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2381. (DEPOSITION ID: 11671).
Refinement Type
Symmetry Type	POINT
Point Symmetry	C1

Map-Model Fitting and Refinement
Id	1 (2BG9)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
Details	METHOD--RIGID BODY REFINEMENT PROTOCOL--ELECTRON MICROSCOPY

Data Acquisition
Detector Type	GATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å**2)	50

Imaging Experiment	1
Date of Experiment	2008-07-25
Temperature (Kelvin)	85
Microscope Model	FEI TECNAI F30
Minimum Defocus (nm)	3000
Maximum Defocus (nm)	6000
Minimum Tilt Angle (degrees)	-70
Maximum Tilt Angle (degrees)	70
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	69000
Calibrated Magnification	80213
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details	DUAL TILT AXIS TOMOGRAPHY

EM Software
Task	Software Package	Version
MODEL FITTING	UCSF Chimera
RECONSTRUCTION	IMOD
RECONSTRUCTION	PRIISM/IVE
RECONSTRUCTION	TOMO3D
RECONSTRUCTION	Xmipp

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.