4BD3
Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | TRIPLE RESONANCE | 90% WATER/10% D2O | 200 mM | 7.0 | 1.0 atm | 298.0 | ||
| 2 | NOESY | 90% WATER/10% D2O | 200 mM | 7.0 | 1.0 atm | 298.0 | ||
| 3 | EDITED -FILTERED | 90% WATER/10% D2O | 200 mM | 7.0 | 1.0 atm | 298.0 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| ARIA | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINT VIOLATION |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 6 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | NONE |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CNS | 1.2 | BRUNGER |
| 2 | structure solution | NMRView | ||
