4APW
Alp12 filament structure
ELECTRON MICROSCOPY
| Sample |
|---|
| FILAMENT STRUCTURE OF AN ACTIN -LIKE PROTEIN, ALP12, IN CLOSTRIDIUM TETANI |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | FILAMENT |
| Vitrification Instrument | |
| Cryogen Name | |
| Sample Vitrification Details | NEGATIVELY STAINED |
| Staining Type | NEGATIVE |
| Staining Material | uranyl acetate |
| Staining Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 5582 |
| Reported Resolution (Å) | 19.7 |
| Resolution Method | |
| Other Details | SINGLE PARTICLE ANALYSIS USING EOS SOFTWARE PACKAGE SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2068. (DEPOSITION ID: 10712). |
| Refinement Type | |
| Symmetry Type | HELICAL |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (3JS6) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | |||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2011-09-06 |
| Temperature (Kelvin) | |
| Microscope Model | HITACHI H7600 |
| Minimum Defocus (nm) | 800 |
| Maximum Defocus (nm) | 5000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 40000 |
| Calibrated Magnification | |
| Source | LAB6 |
| Acceleration Voltage (kV) | 100 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| RECONSTRUCTION | EOS | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| EACH SCANNED IMAGE | ||||
