4ADV

Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit


ELECTRON MICROSCOPY
Sample
E. COLI METHYLTRANSFERASE KSGA BOUND TO THE E. COLI 30S RIBOSOMAL SUBUNIT
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification DetailsVITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- PLUNGER,
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles23343
Reported Resolution (Å)13.5
Resolution Method
Other Details30S RIBOSOMAL SUBUNIT HEAD AND BODY WERE FITTED SEPARATELY AS RIGID BODIES. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2017. (DEPOSITION ID: ...30S RIBOSOMAL SUBUNIT HEAD AND BODY WERE FITTED SEPARATELY AS RIGID BODIES. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2017. (DEPOSITION ID: 10519).
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (2AVY, 1QYR)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement TargetCross-correlation coefficient
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--XRAY
Data Acquisition
Detector TypeGATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TECNAI 20
Minimum Defocus (nm)2000
Maximum Defocus (nm)4000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification62000
Calibrated Magnification82000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging DetailsSEMI-AUTOMATIC DATA ACQUISITION WITH SERIAL EM SCRIPT
EM Software
TaskSoftware PackageVersion
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONIMAGIC5
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PER IMAGE, CTFFIND3