3ZW6

MODEL OF HEXAMERIC AAA DOMAIN ARRANGEMENT OF GREEN-TYPE RUBISCO ACTIVASE FROM TOBACCO.


ELECTRON MICROSCOPY
Sample
NICOTIANA TABACUM RUBISCO ACTIVASE (R294V)
Specimen Preparation
Sample Aggregation StatePARTICLE
Staining TypeNEGATIVE
Staining Materialuranyl acetate
Staining Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles599
Reported Resolution (Å)20
Resolution Method
Other DetailsA MODULE OF ALPHA HELICAL DOMAIN AND ALPHA-BETA DOMAIN OF THE NEIGHBOURING SUBUNIT WAS OVERLAID WITH THE P97 D2 (3CF3) STRUCTURE AND THE HEXAMER WAS F ...A MODULE OF ALPHA HELICAL DOMAIN AND ALPHA-BETA DOMAIN OF THE NEIGHBOURING SUBUNIT WAS OVERLAID WITH THE P97 D2 (3CF3) STRUCTURE AND THE HEXAMER WAS FITTED INTO THE EM MAP SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1940. (DEPOSITION ID: 10169).
Refinement Type
Symmetry TypePOINT
Point SymmetryC6
Map-Model Fitting and Refinement
Id1 (3T15)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement TargetLOCAL MINIMISATION, FIT IN MAP
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeFEI EAGLE (2k x 2k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2011-06-22
Temperature (Kelvin)
Microscope ModelFEI TECNAI 12
Minimum Defocus (nm)470
Maximum Defocus (nm)950
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification
Calibrated Magnification
SourceLAB6
Acceleration Voltage (kV)120
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONIMAGIC
RECONSTRUCTIONMRC IMAGE PROCESSING PACKAGE
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING, EACH PARTICLE