3ZW6
MODEL OF HEXAMERIC AAA DOMAIN ARRANGEMENT OF GREEN-TYPE RUBISCO ACTIVASE FROM TOBACCO.
ELECTRON MICROSCOPY
Sample |
---|
NICOTIANA TABACUM RUBISCO ACTIVASE (R294V) |
Specimen Preparation | |
---|---|
Sample Aggregation State | PARTICLE |
Staining Type | NEGATIVE |
Staining Material | uranyl acetate |
Staining Details |
3D Reconstruction | |
---|---|
Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 599 |
Reported Resolution (Å) | 20 |
Resolution Method | |
Other Details | A MODULE OF ALPHA HELICAL DOMAIN AND ALPHA-BETA DOMAIN OF THE NEIGHBOURING SUBUNIT WAS OVERLAID WITH THE P97 D2 (3CF3) STRUCTURE AND THE HEXAMER WAS F ... |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | C6 |
Map-Model Fitting and Refinement | |||||
---|---|---|---|---|---|
Id | 1 (3T15) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | RIGID BODY FIT | ||||
Refinement Target | LOCAL MINIMISATION, FIT IN MAP | ||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | METHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY |
Data Acquisition | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detector Type | FEI EAGLE (2k x 2k) | ||||||||
Electron Dose (electrons/Å**2) | 20 |
Imaging Experiment | 1 |
---|---|
Date of Experiment | 2011-06-22 |
Temperature (Kelvin) | |
Microscope Model | FEI TECNAI 12 |
Minimum Defocus (nm) | 470 |
Maximum Defocus (nm) | 950 |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | 2 |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | |
Calibrated Magnification | |
Source | LAB6 |
Acceleration Voltage (kV) | 120 |
Imaging Details |
EM Software | ||
---|---|---|
Task | Software Package | Version |
MODEL FITTING | UCSF Chimera | |
RECONSTRUCTION | IMAGIC | |
RECONSTRUCTION | MRC IMAGE PROCESSING PACKAGE | |
RECONSTRUCTION | SPIDER |
Image Processing | ||||
---|---|---|---|---|
CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
PHASE FLIPPING, EACH PARTICLE |