3ZUH

Negative stain EM Map of the AAA protein CbbX, a red-type Rubisco activase from R. sphaeroides


ELECTRON MICROSCOPY
Sample
PROTEIN CBBX
Specimen Preparation
Sample Aggregation StatePARTICLE
Staining TypeNEGATIVE
Staining Materialuranyl acetate
Staining Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles245
Reported Resolution (Å)21
Resolution Method
Other DetailsMODULE CONSISTING OF CHAIN A (204-296) AND CHAIN B (8-203) AS FOUND IN PDB 3SYL WAS MODELLED ONTO D2 P97 OF PDB 3CF3. SUBMISSION BASED ON EXPERIMENTAL ...MODULE CONSISTING OF CHAIN A (204-296) AND CHAIN B (8-203) AS FOUND IN PDB 3SYL WAS MODELLED ONTO D2 P97 OF PDB 3CF3. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1932.
Refinement Type
Symmetry TypePOINT
Point SymmetryC6
Map-Model Fitting and Refinement
Id1 (3SYL)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--MANUAL
Data Acquisition
Detector TypeFEI EAGLE (2k x 2k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2010-11-19
Temperature (Kelvin)
Microscope ModelFEI TECNAI 12
Minimum Defocus (nm)260
Maximum Defocus (nm)1800
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification90600
Calibrated Magnification90600
SourceLAB6
Acceleration Voltage (kV)120
Imaging Details
EM Software
TaskSoftware PackageVersion
RECONSTRUCTIONIMAGIC
RECONSTRUCTIONMRC IMAGE PROCESSING PACKAGE
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING, EACH PARTICLE