3ZEE

Electron cyro-microscopy helical reconstruction of Par-3 N terminal domain

ELECTRON MICROSCOPY

Sample
PAR-3 N-TERMINAL DUF3534 DOMAIN

Specimen Preparation
Sample Aggregation State	FILAMENT
Vitrification Instrument	FEI VITROBOT MARK IV
Cryogen Name	ETHANE
Sample Vitrification Details	LIQUID ETHANE

3D Reconstruction
Reconstruction Method	HELICAL
Number of Particles	84000
Reported Resolution (Å)	6.1
Resolution Method	FSC 0.5 CUT-OFF
Other Details	THE INITIAL MODEL WAS OBTAINED USING IHRSR. THEN THE FINAL RECONSTRUCTION WERE OBTAINED BY PROJECTION MATCHING IN EMAN. THE RESOLUTION CRITERIA USED W ...	THE INITIAL MODEL WAS OBTAINED USING IHRSR. THEN THE FINAL RECONSTRUCTION WERE OBTAINED BY PROJECTION MATCHING IN EMAN. THE RESOLUTION CRITERIA USED WAS GOLDEN CRITERIA FSC 0.5. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2237. (DEPOSITION ID: 11249).
Refinement Type	HALF-MAPS REFINED INDEPENDENTLY
Symmetry Type	HELICAL

Map-Model Fitting and Refinement
Id	1 (4I6P)
Refinement Space	REAL
Refinement Protocol	OTHER
Refinement Target	ENERGY FUNCTION IN NAMD2
Overall B Value
Fitting Procedure
Details	METHOD--CROSS CORRELATION REFINEMENT PROTOCOL--X-RAY

Data Acquisition
Detector Type	GATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment	2010-12-01
Temperature (Kelvin)	95
Microscope Model	FEI TITAN KRIOS
Minimum Defocus (nm)	1800
Maximum Defocus (nm)	2500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2.7
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	96000
Calibrated Magnification
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details	THE MICROSCOPE MODEL IS FEI TITAN KRIOS. 6460 RAW IMAGES WERE COLLECTED AUTOMATICALLY USING THE PACKAGE LEGINON. GOOD MICROGRAPHS WERE SELECTED ONE BY ONE

EM Software
Task	Software Package	Version
MODEL FITTING	NAMD	2
MODEL FITTING	VMD
RECONSTRUCTION	IMAGIC

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	INDIVIDUAL MICROGRAPHS

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.