3J9M
Structure of the human mitochondrial ribosome (class 1)
ELECTRON MICROSCOPY
| Sample |
|---|
| Human mitochondrial ribosome |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK II |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Blot 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK II). |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 884122 |
| Reported Resolution (Å) | 3.5 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | Data were processed in Relion to compensate for beam-induced movement. |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI FALCON II (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 25 | ||||||||
| Imaging Experiment | 1 | 2 | 3 | 4 |
|---|---|---|---|---|
| Date of Experiment | 2014-04-03 | 2014-04-11 | 2014-05-10 | 2014-05-30 |
| Temperature (Kelvin) | 85 | 85 | 85 | 85 |
| Microscope Model | FEI TITAN KRIOS | FEI TITAN KRIOS | FEI TITAN KRIOS | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 1500 | 1500 | 1500 | 1500 |
| Maximum Defocus (nm) | 3500 | 3500 | 3500 | 3500 |
| Minimum Tilt Angle (degrees) | ||||
| Maximum Tilt Angle (degrees) | ||||
| Nominal CS | 2.7 | 2.7 | 2.7 | 2.7 |
| Imaging Mode | BRIGHT FIELD | BRIGHT FIELD | BRIGHT FIELD | BRIGHT FIELD |
| Specimen Holder Model | ||||
| Nominal Magnification | 59000 | 59000 | 59000 | 59000 |
| Calibrated Magnification | 104478 | 104478 | 104478 | 104478 |
| Source | FIELD EMISSION GUN | FIELD EMISSION GUN | FIELD EMISSION GUN | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 | 300 | 300 | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| CTF CORRECTION | CTFFIND | 3 |
| RECONSTRUCTION | RELION | |
