3J9D

Atomic structure of a non-enveloped virus reveals pH sensors for a coordinated process of cell entry


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
f_dihedral_angle_d20.314
f_angle_d1.967
f_chiral_restr0.079
f_bond_d0.027
f_plane_restr0.009
Sample
Bluetongue Virus 1
Sample Components
VP2 of Bluetongue Virus
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsPlunged into liquid ethane (FEI VITROBOT MARK IV).
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles5008
Reported Resolution (Å)3.3
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Data Acquisition
Detector TypeGATAN K2 (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2013-11-29
Temperature (Kelvin)82
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)700
Maximum Defocus (nm)3300
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification14000
Calibrated Magnification24140
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
RECONSTRUCTIONFREALIGN
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
each particle