3J83
Heptameric EspB Rosetta model guided by EM density
ELECTRON MICROSCOPY
| Sample |
|---|
| Mycobacterial protein |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Staining Type | NEGATIVE |
| Staining Material | uranyl formate |
| Staining Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 1455 |
| Reported Resolution (Å) | 30 |
| Resolution Method | FSC 0.5 CUT-OFF |
| Other Details | Initial reconstructions were determined from a side-view 2D average based on observed 7-fold symmetry of the top view using a rotational extrusion met ... |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C7 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (4WJ1) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | RMSD / Rosetta score | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--rigid body DETAILS--Homology model generated from PDB coordinates and used for symmetric docking in Rosetta. | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI EAGLE (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 25 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2013-08-28 |
| Temperature (Kelvin) | 298 |
| Microscope Model | FEI TECNAI SPIRIT |
| Minimum Defocus (nm) | 800 |
| Maximum Defocus (nm) | 1200 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | SIDE ENTRY, EUCENTRIC |
| Nominal Magnification | 49000 |
| Calibrated Magnification | 65900 |
| Source | LAB6 |
| Acceleration Voltage (kV) | 120 |
| Imaging Details | Low dose |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | Rosetta | |
| RECONSTRUCTION | EMAN | 2 |
| RECONSTRUCTION | SPIDER | |
