3J7P

Structure of the 80S mammalian ribosome bound to eEF2


ELECTRON MICROSCOPY
Sample
The 80S-eEF2 complex purified from porcine pancreas
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification Details3 uL sample was incubated on the grid for 30 seconds and blotted for 9 seconds before being plunged into liquid ethane (FEI VITROBOT MARK IV).
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles36667
Reported Resolution (Å)3.5
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (3J3A, 3J3B, 3J3D, 3J3F)
Refinement SpaceRECIPROCAL
Refinement ProtocolOTHER
Refinement TargetR-factor and FSC
Overall B Value37
Fitting Procedure
DetailsMETHOD--Maximum likelihood
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)27
Imaging Experiment1
Date of Experiment2014-04-07
Temperature (Kelvin)70
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)2500
Maximum Defocus (nm)3500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification59000
Calibrated Magnification104478
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGREFMAC
RECONSTRUCTIONRELION
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
Each particle