3J7N

Virus model of brome mosaic virus (second half data set)


ELECTRON MICROSCOPY
Sample
Brome mosaic virus
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsPlunged into liquid ethane (FEI VITROBOT MARK IV).
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles30000
Reported Resolution (Å)3.8
Resolution MethodFSC 0.143 CUT-OFF
Other Details(Single particle--Applied symmetry: I)
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeDIRECT ELECTRON DE-12 (4k x 3k)
Electron Dose (electrons/Å**2)
Imaging Experiment1
Date of Experiment2013-01-10
Temperature (Kelvin)
Microscope ModelJEOL 3200FSC
Minimum Defocus (nm)500
Maximum Defocus (nm)2000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelJEOL 3200FSC CRYOHOLDER
Nominal Magnification50000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
RECONSTRUCTIONEMAN
RECONSTRUCTIONMPSA