3J6C

Cryo-EM structure of MAVS CARD filament

ELECTRON MICROSCOPY

Sample
MAVS CARD filament

Specimen Preparation
Sample Aggregation State	FILAMENT
Vitrification Instrument	FEI VITROBOT MARK III
Cryogen Name	ETHANE
Sample Vitrification Details	Plunged into liquid ethane (FEI VITROBOT MARK III)

3D Reconstruction
Reconstruction Method	HELICAL
Number of Particles
Reported Resolution (Å)	9.6
Resolution Method	FSC 0.5 CUT-OFF
Other Details	Final data were calculated from three separate datasets from three sessions of data collection. The handedness of the map was determined by cryo-elect ...	Final data were calculated from three separate datasets from three sessions of data collection. The handedness of the map was determined by cryo-electron tomography (Helical Details: IHRSR).
Refinement Type
Symmetry Type	HELICAL
Axial Symmetry	C3
Axial Rise	16.8
Angular Rotation	53.6

Map-Model Fitting and Refinement
Id	1 (2VGQ)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
Details	REFINEMENT PROTOCOL--rigid body DETAILS--The docking of one X-ray model into a segmented map corresponding to one subunit was first done manually in ...	REFINEMENT PROTOCOL--rigid body DETAILS--The docking of one X-ray model into a segmented map corresponding to one subunit was first done manually in Chimera, and then optimized using SITUS.

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment	2011-10-20
Temperature (Kelvin)
Microscope Model	JEOL 2200FS
Minimum Defocus (nm)	800
Maximum Defocus (nm)	2000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	GATAN LIQUID NITROGEN
Nominal Magnification	60000
Calibrated Magnification	61950
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	Situs
MODEL FITTING	UCSF Chimera
RECONSTRUCTION	EMAN
RECONSTRUCTION	IMAGIC	4D
RECONSTRUCTION	MRC
RECONSTRUCTION	SPIDER

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	Each filament segment

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.