3J4A
Structure of gp8 connector protein
ELECTRON MICROSCOPY
| Sample |
|---|
| T7 tail complex formed by proteins gp8, gp11 and gp12 |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | LEICA EM CPC |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Samples were applied to grids for 1 minute, blotted and plunged into liquid ethane (LEICA EM CPC). |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 1820 |
| Reported Resolution (Å) | 12 |
| Resolution Method | FSC |
| Other Details | (Single particle--Applied symmetry: C6) |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C12 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (3LJ5) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | Volumetric correlation | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--rigid body DETAILS--One monomer was manually fitted and then the oligomer was generated using SITUS program | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI EAGLE (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 10 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2012-11-08 |
| Temperature (Kelvin) | |
| Microscope Model | FEI TECNAI F20 |
| Minimum Defocus (nm) | 1500 |
| Maximum Defocus (nm) | 3500 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.26 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | GATAN LIQUID NITROGEN |
| Nominal Magnification | 108696 |
| Calibrated Magnification | 108696 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | UCSF Chimera | |
| RECONSTRUCTION | EMAN | |
| RECONSTRUCTION | SPIDER | |
| RECONSTRUCTION | Xmipp | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| each micrograph | ||||
