3J48

Cryo-EM structure of Poliovirus 135S particles

ELECTRON MICROSCOPY

Sample
Poliovirus 1 Mahoney 135S particle

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	HOMEMADE PLUNGER
Cryogen Name	ETHANE
Sample Vitrification Details	Blotted manually in ambient atmosphere before plunging into ethane cooled by liquid nitrogen.

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	117330
Reported Resolution (Å)	5.5
Resolution Method	FSC 0.143 CUT-OFF
Other Details	Single particle details: The particles were selected using an automatic selection program (Single particle--Applied symmetry: I)
Refinement Type
Symmetry Type	POINT
Point Symmetry	I

Map-Model Fitting and Refinement
Id	1 (1POV, 1POV, 1POV)
Refinement Space	RECIPROCAL
Refinement Protocol	RIGID BODY FIT
Refinement Target	mean amplitude-weighted cosine of the phase difference
Overall B Value
Fitting Procedure
Details	METHOD--rigid body REFINEMENT PROTOCOL--rigid body DETAILS--Most of the model was docked, with specific areas of discrepancy fitted. The fitting was ...	METHOD--rigid body REFINEMENT PROTOCOL--rigid body DETAILS--Most of the model was docked, with specific areas of discrepancy fitted. The fitting was rigid body with flexible fitting or deletion of selected polypeptide segments. Rigid bodies for VP1, VP2, VP3, and the VP3 beta tube were defined to include beta barrels and non-covalently attached polypeptides. Each rigid body was repeatedly fitted manually and then refined. Disordered polypeptide segments were removed. Several rearranged segments were included as approximate backbone traces and refined.

Data Acquisition
Detector Type	TVIPS TEMCAM-F415 (4k x 4k)
Electron Dose (electrons/Å**2)	15

Imaging Experiment	1
Date of Experiment	2011-02-28
Temperature (Kelvin)	90
Microscope Model	FEI TECNAI F20
Minimum Defocus (nm)	980
Maximum Defocus (nm)	2000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	GATAN LIQUID NITROGEN
Nominal Magnification	62000
Calibrated Magnification
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	Coot
MODEL FITTING	REFMAC
RECONSTRUCTION	FREALIGN

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	Each micrograph

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.