3J3X
Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)
ELECTRON MICROSCOPY
| Sample |
|---|
| Mm-cpn with 1mM ATP/AlFx |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK III |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Blotted once for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III) |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 22571 |
| Reported Resolution (Å) | 4.3 |
| Resolution Method | FSC 0.5 CUT-OFF |
| Other Details | (Single particle--Applied symmetry: D8) |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | D8 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (1Q3Q) | ||||
| Refinement Space | |||||
| Refinement Protocol | |||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN ULTRASCAN 4000 (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 20 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2008-02-29 |
| Temperature (Kelvin) | 100 |
| Microscope Model | JEOL 3200FSC |
| Minimum Defocus (nm) | 1000 |
| Maximum Defocus (nm) | 3000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 4.1 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | JEOL 3200FSC CRYOHOLDER |
| Nominal Magnification | 80000 |
| Calibrated Magnification | 112000 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| RECONSTRUCTION | EMAN | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| Each micrograph | ||||
