3J3S

Structural dynamics of the MecA-ClpC complex revealed by cryo-EM


ELECTRON MICROSCOPY
Sample
MecA-ClpC(E618A)
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles41902
Reported Resolution (Å)11
Resolution MethodFSC 0.5 CUT-OFF
Other Details(Single particle--Applied symmetry: C6)
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (3PXI)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement TargetCross-correlation
Overall B Value
Fitting Procedure
DetailsREFINEMENT PROTOCOL--flexible DETAILS--Protocol- Initial local fitting was done using Chimera and then MDFF was used for flexible fitting. ref- Trabu ...REFINEMENT PROTOCOL--flexible DETAILS--Protocol- Initial local fitting was done using Chimera and then MDFF was used for flexible fitting. ref- Trabuco, L.G., Villa, E., Mitra, K., Frank, J. and Schulten, K. (2008) Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics.
Data Acquisition
Detector TypeFEI EAGLE (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2010-09-11
Temperature (Kelvin)90
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1500
Maximum Defocus (nm)3000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification59000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGMDFF
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
each defocus group on 3D level