3J30
Electron Cryo-microscopy of Chikungunya VLP in complex with neutralizing antibody Fab CHK152
ELECTRON MICROSCOPY
| Sample |
|---|
| Electron Cryo-microscopy of Chikungunya VLP in complex with neutralizing antibody Fab CHK15 2 |
| Sample Components |
| CHK152 antibody Fab fragment |
| Chikungunya virus like particle |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | GATAN CRYOPLUNGE 3 |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | Blot for 2 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3) |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 2106 |
| Reported Resolution (Å) | 16 |
| Resolution Method | FSC 0.5 CUT-OFF |
| Other Details | (Single particle details: The particles were selected using e2boxer) (Single particle--Applied symmetry: I) |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (4GQ8) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | Sumf | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--rigid body | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | |||||||||
| Electron Dose (electrons/Å**2) | 20 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2011-04-01 |
| Temperature (Kelvin) | |
| Microscope Model | FEI/PHILIPS CM200FEG |
| Minimum Defocus (nm) | |
| Maximum Defocus (nm) | |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | GATAN LIQUID NITROGEN |
| Nominal Magnification | 35000 |
| Calibrated Magnification | 35600 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | EMfit | |
| RECONSTRUCTION | EMAN | 2 |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| Each micrograph | ||||
