3J2G

Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM


ELECTRON MICROSCOPY
Sample
immature ribosomal small subunit from rimm gene deleted E.coli strain treated with RimM in vitro
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles25631
Reported Resolution (Å)16.5
Resolution MethodFSC 0.5 CUT-OFF
Other DetailsDetails about the particle: This is a classification volume (No. 4) using ML3D methods.
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (3OFA)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement TargetCross-correlation
Overall B Value
Fitting Procedure
DetailsREFINEMENT PROTOCOL--Initial local fitting was done using Chimera and then MDFF was used for flexible fitting DETAILS--ref- Trabuco, L.G., Villa, E., ...REFINEMENT PROTOCOL--Initial local fitting was done using Chimera and then MDFF was used for flexible fitting DETAILS--ref- Trabuco, L.G., Villa, E., Mitra, K., Frank, J. and Schulten, K. (2008) Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics. Structure, 16, 673-683
Data Acquisition
Detector TypeFEI EAGLE (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2012-01-01
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1500
Maximum Defocus (nm)3800
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelGATAN LIQUID NITROGEN
Nominal Magnification59000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGMDFF
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
Weiner filter