3IYH
P22 procapsid coat protein structures reveal a novel mechanism for capsid maturation: Stability without auxiliary proteins or chemical cross-links
ELECTRON MICROSCOPY
| Sample |
|---|
| P22 Heat Expanded Heads |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 3308 |
| Reported Resolution (Å) | 8.2 |
| Resolution Method | FSC 0.5 CUT-OFF |
| Other Details | Final map was calculated at 7.0A resolution ( Details about the particle: data were collected on film ) |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (1OHG) | 2 (1FHG) | |||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | cross-correlation coefficient | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--local refinement, flexible fitting REFINEMENT PROTOCOL--rigid body | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 8 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2009-01-01 |
| Temperature (Kelvin) | 90 |
| Microscope Model | FEI POLARA 300 |
| Minimum Defocus (nm) | 630 |
| Maximum Defocus (nm) | 3160 |
| Minimum Tilt Angle (degrees) | -9999 |
| Maximum Tilt Angle (degrees) | -9999 |
| Nominal CS | 2.3 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | OTHER |
| Nominal Magnification | 39000 |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | Coot | |
| MODEL FITTING | MODELLER | |
| RECONSTRUCTION | Auto3DEM | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| ROBEM | ||||
