3IY0
Variable domains of the x-ray structure of Fab 14 fitted into the cryoEM reconstruction of the virus-Fab 14 complex
ELECTRON MICROSCOPY
| Sample |
|---|
| FAB FRAGMENT OF MAB14 INTERACTING WITH CANINE PARVOVIRUS |
| Sample Components |
| canine parvovirus |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 2059 |
| Reported Resolution (Å) | 12.5 |
| Resolution Method | |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 37 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2004-06-17 |
| Temperature (Kelvin) | 93 |
| Microscope Model | FEI/PHILIPS CM300FEG/T |
| Minimum Defocus (nm) | 1 |
| Maximum Defocus (nm) | 3.8 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 45000 |
| Calibrated Magnification | 47190 |
| Source | TUNGSTEN HAIRPIN |
| Acceleration Voltage (kV) | 45 |
| Imaging Details |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| ROBEM | ||||
