3EZK

Bacteriophage T4 gp17 motor assembly based on crystal structures and cryo-EM reconstructions

ELECTRON MICROSCOPY

Sample
T4 procapsid with gp17 bound

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	HOMEMADE PLUNGER
Cryogen Name	ETHANE
Sample Vitrification Details	flash-frozen on holey grids in liquid ethane

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	1716
Reported Resolution (Å)	34
Resolution Method
Other Details
Refinement Type
Symmetry Type	POINT
Point Symmetry	C5

Map-Model Fitting and Refinement
Id	1 (3CPE)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target	Sumf
Overall B Value
Fitting Procedure
Details	METHOD--N- and C-terminal domains were separately fitted into their corresponding cryoEM densities REFINEMENT PROTOCOL--Rigid body

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment
Temperature (Kelvin)
Microscope Model	FEI/PHILIPS CM200FEG
Minimum Defocus (nm)	2000
Maximum Defocus (nm)	3500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	38000
Calibrated Magnification
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	EMfit
RECONSTRUCTION	SPIDER

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	phase flipping of each micrograph

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.