3EQ4
Model of tRNA(Leu)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
ELECTRON MICROSCOPY
Sample |
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ribosome in pre-accommodated state |
Specimen Preparation | |
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Sample Aggregation State | PARTICLE |
Vitrification Instrument | FEI VITROBOT MARK I |
Cryogen Name | NITROGEN |
Sample Vitrification Details |
3D Reconstruction | |
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Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 80000 |
Reported Resolution (Å) | 12 |
Resolution Method | |
Other Details | |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | C1 |
Map-Model Fitting and Refinement | |||||
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Id | 1 (2AVY, 2AW4, 1QZA, 1OB2) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | OTHER | ||||
Refinement Target | cross-correlation coefficient | ||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | METHOD--See Method in the citation REFINEMENT PROTOCOL--auto |
Data Acquisition | |||||||||
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Detector Type | GENERIC CCD | ||||||||
Electron Dose (electrons/Å**2) | 15 |
Imaging Experiment | 1 |
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Date of Experiment | 2005-07-15 |
Temperature (Kelvin) | |
Microscope Model | FEI TECNAI F20 |
Minimum Defocus (nm) | 1000 |
Maximum Defocus (nm) | 4000 |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | 50000 |
Calibrated Magnification | |
Source | FIELD EMISSION GUN |
Acceleration Voltage (kV) | 300 |
Imaging Details |
EM Software | ||
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Task | Software Package | Version |
MODEL FITTING | RSRef | |
RECONSTRUCTION | SPIDER |