3DG4

Coordinates of 16S and 23S rRNAs fitted into the cryo-EM map of RF1-bound termination complex

ELECTRON MICROSCOPY

Sample
E. coli 70S ribosome bound with RF1

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	HOMEMADE PLUNGER
Cryogen Name	ETHANE
Sample Vitrification Details	Rapid freezing in liquid ethane

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	24622
Reported Resolution (Å)	12.8
Resolution Method
Other Details	SPIDER package
Refinement Type
Symmetry Type	POINT
Point Symmetry	C1

Map-Model Fitting and Refinement
Id	1 (2AVY, 2AW4)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target	cross-correlation coefficient, real-space R factor
Overall B Value
Fitting Procedure
Details	METHOD--Auto REFINEMENT PROTOCOL--multi-rigid body, real-space refinement

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1
Date of Experiment	2002-12-01
Temperature (Kelvin)	93
Microscope Model	FEI TECNAI F20
Minimum Defocus (nm)	2000
Maximum Defocus (nm)	4000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	50000
Calibrated Magnification	49696
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	RSRef
RECONSTRUCTION	SPIDER

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	CTF correction of 3D map

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.