2KZL

Specifier domain and GA motif region of B. subtilis tyrS T box leader RNA


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D 1H-13C HSQC1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
23D HCCH-TOCSY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
32D 1H-1H NOESY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
43D 1H-13C NOESY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
52D 1H-15N HSQC1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA90% H2O/10% D2O6.8288
63D 1H-15N NOESY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA90% H2O/10% D2O6.8288
72D 1H-13C HSQC1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
83D HCCH-COSY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
93D HCN1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
102D 1H-1H NOESY1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA90% H2O/10% D2O6.8288
112D 1H-15N HSQC1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
122D 1H-13C ct-spin-echo difference1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA100% D2O6.8ambient301
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA600
2VarianINOVA800
NMR Refinement
MethodDetailsSoftware
simulated annealingVnmrJ
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations
Conformers Calculated Total Number50
Conformers Submitted Total Number8
Representative Model1 (closest to the average)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionVnmrJVarian