Experiment: 2KZL

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-13C HSQC	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
2	3D HCCH-TOCSY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
3	2D 1H-1H NOESY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
4	3D 1H-13C NOESY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
5	2D 1H-15N HSQC	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	90% H2O/10% D2O		6.8		288
6	3D 1H-15N NOESY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	90% H2O/10% D2O		6.8		288
7	2D 1H-13C HSQC	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
8	3D HCCH-COSY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
9	3D HCN	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
10	2D 1H-1H NOESY	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	90% H2O/10% D2O		6.8		288
11	2D 1H-15N HSQC	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301
12	2D 1H-13C ct-spin-echo difference	1 mM [U-100% 13C; U-100% 15N] Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	100% D2O		6.8	ambient	301

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	600
2	Varian	INOVA	800

NMR Refinement
Method	Details	Software
simulated annealing		VNMRJ

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations
Conformers Calculated Total Number	50
Conformers Submitted Total Number	8
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	VNMRJ		Varian

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.