NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
2 | 2D 1H-13C-CT-HSQC | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
3 | 3D CBCA(CO)NH | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
4 | 3D C(CO)NH | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
5 | 3D HNCA | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
6 | 3D HNCACB | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
7 | 3D H(CCO)NH | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
8 | 3D HCCH-COSY | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
9 | 3D 1H-13C NOESY | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
10 | 3D 1H-15N NOESY | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
11 | 2D 1H-13C-CT-HSQC_aromatics | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
12 | 2D (HB)CB(CGCDCE)HE | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
13 | 2D (HB)CB(CGCD)HD | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
14 | 3D HN(CO)CA | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
15 | 3D HNCO | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
16 | 3D HCCH-TOCSY | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
17 | HN(CA)CO | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |
18 | HNCAHA | 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide | 93% H2O/7% D2O | 100 | 6.8 | ambient | 303 | |