2YUO

Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1.00mM Protein; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3 ;0.05mM ZnCl2, 1mM IDA; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient296
23D_13C-separated_NOESY1.00mM Protein; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3 ;0.05mM ZnCl2, 1mM IDA; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient296
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR3.5Bruker
2processingNMRPipe20031121Delaglio, F.
3data analysisNMNMRView5.0.4Johnson, B.A.
4data analysisKUJIRA0.9747Kobayashi, N.
5structure solutionCYANA2.0.17Guntert, P.
6refinementCYANA2.0.17Guntert, P.