2YRQ

Solution structure of the tandem HMG box domain from Human High mobility group protein B1


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.09mM HMG box domain U-15N,13C; 20mM d-Tris-HCl (pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient296
23D_15N-separated_NOESY1.09mM HMG box domain U-15N,13C; 20mM d-Tris-HCl (pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient296
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE II900
NMR Refinement
MethodDetailsSoftware
torsion angle dynamics, restrainted molecular dynamicsTopSpin
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, structures with the lowest energy, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionTopSpin1.3Bruker
2processingNMRPipe20060524Delaglio, F.
3data analysisNMRView5.0.4Johnson, B.A.
4data analysisKUJIRA0.9823Kobayashi, N.
5structure solutionCYANA2.0.17Guntert, P.
6refinementCYANA2.0.17Guntert, P.