2VXF

Solution structure of the LSm-domain of zebrafish RAP55


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-SEPARATED_NOESY100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
23D_13C-SEPARATED_NOESY100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
3HNCO100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
4HNCACB100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
5CCCONH100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
6HCCCONH100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
7CBCACONH100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
8HBHACONH100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
9HCH-TOCSY100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
10CCH- TOCSY100 MM NACL, 10 MM BIS- TRIS, PH 7.0, 5 MM DTT, 90% H2O, 10% D2O100 mM7.01.0 atm289.0
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA600
NMR Refinement
MethodDetailsSoftware
XPLORREFINEMENT AS BEEN DONE SIMILARLY TO THE N-TERMINAL LSM DOMAIN OF TRALX-PLOR
NMR Ensemble Information
Conformer Selection CriteriaMINIMUM ENERGY
Conformers Calculated Total Number50
Conformers Submitted Total Number20
Representative Model1 (n/a)
Additional NMR Experimental Information
DetailsNONE
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementX-PLOR2.9.4BRUNGER
2structure solutionSparky