2TPK
AN INVESTIGATION OF THE STRUCTURE OF THE PSEUDOKNOT WITHIN THE GENE 32 MESSENGER RNA OF BACTERIOPHAGE T2 USING HETERONUCLEAR NMR METHODS
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 6.5 | 293 | |||||
| 2 | DQFCOSY | 6.5 | 293 | |||||
| 3 | TOCSY | 6.5 | 293 | |||||
| 4 | NOESY-TOCSY | 6.5 | 293 | |||||
| 5 | CT-HSQC | 6.5 | 293 | |||||
| 6 | HCCH-COSY | 6.5 | 293 | |||||
| 7 | NOESY-HMQC | 6.5 | 293 | |||||
| 8 | HMQC | 6.5 | 293 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | 500 | |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY-SIMULATED ANNEALING AND ENERGY MINIZATION PROTOCOLS | REFINEMNET DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | 19 |
| Conformers Submitted Total Number | 1 |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING NMR SPECTROSCOPY ON 13C,15N-LABELED AND SELECTIVELY DEUTERATED T2 PSEUDOKNOT SAMPLE |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.1 | BRUNGER |
| 2 | structure solution | Felix | ||
| 3 | structure solution | XPLOR | 3.1 | BRUNGER |
