2RS7
Solution structure of the second dsRBD from RNA helicase A
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D 1H-13C NOESY | 1.16 mM [U-100% 13C; U-100% 15N] entity-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 1 mM DTT-4, 0.02 % sodium azide-5 | 90% H2O/10% D2O | 120 | 6.0 | ambient | 298 | |
| 2 | 3D 1H-15N NOESY | 1.16 mM [U-100% 13C; U-100% 15N] entity-1, 20 mM sodium phosphate-2, 100 mM sodium chloride-3, 1 mM DTT-4, 0.02 % sodium azide-5 | 90% H2O/10% D2O | 120 | 6.0 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 800 |
| 2 | Bruker | AVANCE | 700 |
| 3 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing, torsion angle dynamics | Amber | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (fewest violations) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | Amber | Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, Kollm | |
| 2 | refinement | CYANA | ||
