2RPC

Solution structure of the tandem zf-C2H2 domains from the human zinc finger protein ZIC 3

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D 1H-15N NOESY	0.57mM [U-13C; U-15N] zinc finger protein ZIC 3; 20mM [U-2H] TRIS; 100mM sodium chloride; 1mM [U-2H] DTT; 0.02 % sodium azide; 0.05mM ZnCl2; 1mM IDA; 10% [U-2H] D2O; 90% H2O/10% D2O	90% H2O/10% D2O	120	7.0	ambient	298
2	3D 1H-13C NOESY	0.57mM [U-13C; U-15N] zinc finger protein ZIC 3; 20mM [U-2H] TRIS; 100mM sodium chloride; 1mM [U-2H] DTT; 0.02 % sodium azide; 0.05mM ZnCl2; 1mM IDA; 10% [U-2H] D2O; 90% H2O/10% D2O	90% H2O/10% D2O	120	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	900

NMR Refinement
Method	Details	Software
torsion angle dynamics		TopSpin

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (fewest violations)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	TopSpin		Bruker Biospin
2	processing	NMRPipe		Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
3	data analysis	NMRView		Johnson, One Moon Scientific
4	data analysis	KUJIRA		Kobayashi, N
5	structure solution	CYANA		Guntert, Mumenthaler and Wuthrich
6	refinement	CYANA		Guntert, Mumenthaler and Wuthrich

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.