Experiment: 2R6R

X-RAY DIFFRACTION

Crystalization Experiments
ID	Method	pH	Temperature	Details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	15 g/L FtsZ, 2 mM MgCl2, 1 mM GDP, 0.1 M MOPS-NaOH, 0.2 M NaCl, 28% (v/v) PEG400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystal Properties
Matthews coefficient	Solvent content
1.91	35.67

Symmetry
Space Group	P 1 21 1

Diffraction Experiment
ID #	Crystal ID	Scattering Type	Data Collection Temperature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1	1	x-ray	100	IMAGE PLATE	MAR scanner 345 mm plate		2006-08-01	M	SINGLE WAVELENGTH

Radiation Source
ID #	Source	Type	Wavelength List	Synchrotron Site	Beamline
1	ROTATING ANODE	RIGAKU	1.54

Overall
ID #	Resolution (High)	Resolution (Low)	Percent Possible (Observed)	R Merge I (Observed)	R Sym I (Observed)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1	1.7	30		0.046	0.046	16.4	2.7		30532	2	2	18.3

Statistics
Diffraction ID	Structure Solution Method	Cross Validation method	Starting model	Resolution (High)	Resolution (Low)	Number Reflections (Observed)	Number Reflections (R-Free)	Percent Reflections (Observed)	R-Factor (Observed)	R-Work	R-Free	R-Free Selection Details	Mean Isotropic B
X-RAY DIFFRACTION	MOLECULAR REPLACEMENT	THROUGHOUT	1FSZ	1.7	20	28538	1486	98.34	0.18104	0.17865	0.22691	RANDOM	16.676

Temperature Factor Modeling
Anisotropic B[1][1]	Anisotropic B[1][2]	Anisotropic B[1][3]	Anisotropic B[2][2]	Anisotropic B[2][3]	Anisotropic B[3][3]
		0.03	0.02		-0.01

RMS Deviations
Key	Refinement Restraint Deviation
r_dihedral_angle_2_deg	35.445
r_dihedral_angle_4_deg	17.746
r_dihedral_angle_3_deg	13.433
r_dihedral_angle_1_deg	5.759
r_scangle_it	4.152
r_scbond_it	2.426
r_angle_refined_deg	1.418
r_mcangle_it	1.32
r_mcbond_it	0.852
r_nbtor_refined	0.306

RMS Deviations
Key	Refinement Restraint Deviation
r_dihedral_angle_2_deg	35.445
r_dihedral_angle_4_deg	17.746
r_dihedral_angle_3_deg	13.433
r_dihedral_angle_1_deg	5.759
r_scangle_it	4.152
r_scbond_it	2.426
r_angle_refined_deg	1.418
r_mcangle_it	1.32
r_mcbond_it	0.852
r_nbtor_refined	0.306
r_nbd_refined	0.214
r_symmetry_vdw_refined	0.203
r_symmetry_hbond_refined	0.187
r_xyhbond_nbd_refined	0.14
r_chiral_restr	0.087
r_bond_refined_d	0.012
r_gen_planes_refined	0.005
r_bond_other_d
r_angle_other_deg
r_gen_planes_other
r_nbd_other
r_nbtor_other
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_vdw_other
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_mcbond_other
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded