2QMV
High Resolution Structure of Peroxisone Proliferation-Activated Receptor gamma and Characterisation of its Interaction with the Co-activator Transcriptional Intermediary Factor 2
SOLUTION NMR
NMR Experiment | ||||||||
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D_15N-separated_NOESY | |||||||
2 | 3D_13C-separated_NOESY | |||||||
3 | CNH-NOESY |
NMR Spectrometer Information | |||
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Spectrometer | Manufacturer | Model | Field Strength |
1 | Bruker | DRX | 600 |
2 | Bruker | DRX | 900 |
NMR Refinement | ||
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Method | Details | Software |
simulated annealing | total of 3017 restraints, 2352 are NOE-derived distance constraints, 439 dihedral angle restraints,226 distance restraints from hydrogen bonds. | DYANA |
NMR Ensemble Information | |
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Conformer Selection Criteria | structures with the lowest energy |
Conformers Calculated Total Number | 1024 |
Conformers Submitted Total Number | 9 |
Representative Model | 1 (fewest violations) |
Computation: NMR Software | ||||
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# | Classification | Version | Software Name | Author |
1 | structure solution | DYANA | 1.5 | Guentert P. |
2 | refinement | DYANA | 1.5 | Guentert P. |