2O10

Solution structure of the N-terminal LIM domain of MLP/CRP3


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1mM N-terminal LIM domain, 20mM phosphate buffer K, 90% H2O, 10% D2O90% H2O/10% D2O150mM KCl6.8ambient295
23D_13C-separated_NOESY1mM N-terminal LIM domain, 20mM phosphate buffer K, 90% H2O, 10% D2O90% H2O/10% D2O150mM KCl6.8ambient295
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
NMR Refinement
MethodDetailsSoftware
simulated annealingzinc coordination fixed by explicit bonds and anglesCNS
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number200
Conformers Submitted Total Number19
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementCNSBRUNGER, ADAMS, CLORE, GROS, NILGES AND READ